Sequoia Pitch Moth in Pines

This fact sheet focuses on the sequoia pitch moth (SPM) (Sesiidae: Synanthedon sequoiae). It includes how to identify, life cycle, damage, and management

Interindividual methylomic variation across blood, cortex, and cerebellum: implications for epigenetic studies of neurological and neuropsychiatric phenotypes

Given the tissue-specific nature of epigenetic processes, the assessment of disease-relevant tissue is an important consideration for epigenome-wide association studies (EWAS). Little is known about whether easily accessible tissues, such as whole blood, can be used to address questions about interindividual epigenomic variation in inaccessible tissues, such as the brain. We quantified DNA methylation in matched DNA samples isolated from whole blood and 4 brain regions (prefrontal cortex, entorhinal cortex, superior temporal gyrus, and cerebellum) from 122 individuals. We explored co-variation between tissues and the extent to which methylomic variation in blood is predictive of interindividual variation identified in the brain. For the majority of DNA methylation sites, interindividual variation in whole blood is not a strong predictor of interindividual variation in the brain, although the relationship with cortical regions is stronger than with the cerebellum. Variation at a subset of probes is strongly correlated across tissues, even in instances when the actual level of DNA methylation is significantly different between them. A substantial proportion of this co-variation, however, is likely to result from genetic influences. Our data suggest that for the majority of the genome, a blood-based EWAS for disorders where brain is presumed to be the primary tissue of interest will give limited information relating to underlying pathological processes. These results do not, however, discount the utility of using a blood-based EWAS to identify biomarkers of disease phenotypes manifest in the brain. We have generated a searchable database for the interpretation of data from blood-based EWAS analyses (http://epigenetics.essex.ac.uk/ bloodbrain/)

The Cerebellar Fastigial Nucleus Contributes to CO\u3csub\u3e2\u3c/sub\u3e-H\u3csup\u3e+\u3c/sup\u3e Ventilatory Sensitivity in Awake Goats

The purpose of this study was to test the hypothesis that an intact cerebellar fastigial nucleus (CFN) is an important determinant of CO2-H+ sensitivity during wakefulness. Bilateral, stainless steel microtubules were implanted into the CFN (N = 9) for injection (0.5–10 μl) of the neurotoxin ibotenic acid. Two or more weeks after implantation of the microtubules, eupneic breathing and CO2-H+ sensitivity did not differ significantly (P \u3e 0.10) from pre-implantation conditions. Injection of ibotenic acid (50 mM) did not significantly alter eupneic PaCO2 (P \u3e 0.10). The coefficient of variation of eupneic PaCO2 was 4.0 ± 0.6 and 3.7 ± 0.4% over the 2 weeks before and after the lesion, respectively. CO2-H+ sensitivity expressed as inspired ventilation/PaCO2 decreased from 2.15 ± 0.17 pre-lesion to 1.58 ± 0.26 l/(min mmHg) 3–6 days post-lesion (P \u3c 0.02, −27%). There was no significant (P \u3e 0.10) recovery of sensitivity between 7 and 10 days post-lesion. The lesion also increased (P \u3c 0.05) the day-to-day variability of this index by nearly 100%. When CO2 sensitivity was expressed as elevated inspired CO2/room air VI, values at 7%, but not 3 and 5% inspired CO2, were reduced and more variable (P \u3c 0.05) after the ibotenic acid injections. We conclude that during wakefulness, the CFN contributes relatively more to overall ventilatory drive at high relative to low levels of hypercapnia

A putative serine protease, SpSsp1, from Saprolegnia parasitica is recognised by sera of rainbow trout, Oncorhynchus mykiss

Acknowledgements Our work was supported by the BBSRC (BB/C518457/1, BB/G012075/1, BB/J018333/1) (K.L.M., C.J.S., J.S.C., K.S.D., and P.v.W.), the University of Aberdeen (V.L.A., C.J.S., and P.v.W.), MSD Animal Health (J.S.C., K.S.D., and A.H.v.d.B), and The Royal Society (P.v.W.). This work was also supported by a Marie Curie Initial Training Networks with the SAPRO (sustainable approaches to reduce Oomycete (Saprolegnia) infections in aquacultures) grant PITN-GA-2009-238550 (A.H.v.d.B., L.L., C.J.S., P.v.W.). We would like to acknowledge Aberdeen Proteomics for carrying out LC–MS/MS and Laura Grenville-Briggs for valuable discussion and technical help. We are grateful to the Broad Institute (Carsten Russ, Rays Jiang, Brian Haas, and Chad Nusbaum), Brett Tyler (VBI), and P.v.W. for early release of draft supercontigs of the genome sequence of isolate CBS233.65, which helped us identify SpSsp1.Peer reviewedPublisher PD

Depredation Impact of Double-Crested Cormorants (Phalacrocorax auritus) on Commercial Catfish Production in the Mississippi Delta

Double-crested Cormorants (Phalacrocorax auritus) impact United States commercial aquaculture and are considered the greatest avian predators on catfish (Ictalurus spp.) aquaculture facilities. Cormorants are especially problematic in the Delta region in western Mississippi, where catfish production is concentrated providing ideal wintering and foraging areas. Although cormorant/aquaculture dynamics have been studied, recent changes in aquaculture practices, regulatory policies, and decreased overall hectares in production merit contemporary research. Therefore, we estimated abundance and distribution of cormorants at their night roosts and assessed diet related to catfish consumption. Aerial surveys of cormorant night roosts were flown from October through April, 2016-2018. Following each survey, three active night roosts were randomly selected for harvesting cormorants for later necropsy and stomach contents assessment. We completed 25 total surveys and counted an average of 23,379 cormorants (range 5,026 to 40,535) pooled over years (corrected for observer and method bias). A total of 728 cormorants from 27 different night roosts were collected across years. Survey count models estimated 4.2 and 5 million cormorant forage days in the Delta during winters 2016-2017 and 2017-2018, respectively. Throughout the study, catfish comprised 33% of the prey biomass detected; shad (Dorosoma spp.) also were dominant (58%) prey. Evidence suggests that the area of catfish aquaculture surrounding a night roost within a 30.6-km forage buffer is an important predictor for a bird’s relative amount of catfish consumption. These results will inform wildlife managers regarding relationships between cormorant night roost locations in the Delta and disproportionate consumption of catfish, enhancing techniques to reduce fish losses on aquaculture facilities

Predicting consistent foraging ecologies of migrating waterbirds: Using stable isotope and parasite measurements as indicators of landscape use

The emergence of novel human pathogens is frequently linked with zoonotic events and human-wildlife interactions that promote disease transmission. Consequently, surveillance of wildlife populations for candidate diseases that could spread to humans is beneficial, but requires widespread collections of numerous samples. A legitimate means to acquire large sample sizes of waterfowl is through cooperation between researchers and hunters, who also work in concert with natural resource managers, landowners, and agricultural entities -e.g., aquaculture facilities. In addition to understanding the occurrence and spread of parasites and pathogens by birds, these samples can be used to answer questions about the ecology of various waterbird species. Body mass and morphometric data on hunter-donated specimen are useful for understanding bird condition and other dynamics of birds; however, when breast meat is removed prior to the acquisition of specimen weight, samples might not be as desirable. Here, we evaluate the utility of data obtained from a bird species that might be targeted by hunters and subsequently used to learn about their disease dynamics. Lesser Scaup (Aythya affinis) collected at aquaculture facilities were assessed for their stable isotope concentrations and parasites communities to learn about the birds’ foraging ecology. Discriminant analyses designed to classify birds by the aquaculture pond type from which they were collected included isotope data, Principal Components derived from parasite community data of 7 types, and birds’ body mass. We compared these to Double-crested Cormorants (Nannopterum auritum) feeding on catfish and found the two waterbird species exhibited different infracommunities of parasites Furthermore, some scaup demonstrated fish aquaculture pond type fidelity. Bird body mass was an important metric to include in analytical models when all parasite datatypes were not available. However, the combination of stable isotope concentrations and parasite infracommunity data (that includes prevalence, abundance, volume, and energy use) in models resulted in host ecology differentiation equal or better than models where bird body mass was included. Hunter-derived samples should be encouraged as a means for sample acquisition and be considered as an approach for aquaculture-wildlife conflict management as the information that can be obtained through these samples is multifaceted

Scaup Depredation on Arkansas Baitfish and Sportfish Aquaculture

Lesser scaup (Aythya affinis) and greater scaup (A. marila), hereafter scaup, consume a variety of aquatic invertebrates, plants, and occasionally small fish. Scaup have foraged on commercial aquaculture farms in the southern United States for decades. However, the types, abundance, and rate of fish exploitation by scaup on baitfish and sportfish farms are not well documented. Thus, information is needed to understand how fish and other foods influence scaup use of aquatic resources, and any potential economic effects of depredation of fish. From November–March in winters 2016–2017 and 2017–2018, we conducted 1,458 pond surveys to estimate the abundance and distribution of scaup on Arkansas baitfish and sportfish farms that commercially produce species such as golden shiners (Notemigonus crysoleucas), fathead minnows (Pimephales promelas), goldfish (Carassius auratus), and sunfish (Lepomis spp.). We also collected and processed 531 foraging scaup and quantified the proportion of scaup consuming fish and the proportion of their diet obtained from fish. Fish consumption was highly variable between years. In our survey area, we estimated total fish consumption at 1,400 kg and 60,500 kg for winters 2016–2017 and 2017–2018, respectively. Sunfish ponds experienced the maximum loss (18,000 fish/ha) during winter 2017–2018, while goldfish ponds experienced a loss of just 2,600 fish/ha during the same winter. The estimates of baitfish and sportfish loss to scaup revealed potential management strategies for minimizing fish loss and can inform economic analysis of the financial impact of scaup on producers

Foraging Ecology and Distribution of Scaup (Aythya spp.) on Arkansas Commercial Baitfish and Sportfish Farms

Lesser Scaup (Aythya affinis)and Greater Scaup (Aythya marila) have been reported to consume substantial quantities of golden shiners (Notemigonus crysoleucas), fathead minnows (Pimephales promelas), goldfish (Carassius auratus), and sunfish (Lepomis spp.) produced on Arkansas commercial baitfish and sportfish farms. The goals of this study were to investigate foraging ecology and distribution of Scaup at these facilities, and use this information to assist producers in administering bird harassment efforts more efficiently. During typical wintering period for Scaup in Arkansas (November-March), we conducted approximately 1,400 pond surveys to estimate abundance and distribution of scaup on farms in 2016-2017 and 2017-2018. Information related to pond size, fish species, fish size, and stocking density, were also obtained to enable a more detailed analysis of Scaup use. We also collected 561 Scaup from these facilities to quantify the proportion of diet obtained from fish. There was an increase in Scaup abundance and fish consumption between the first to the second winter, likely attributed to cooler temperatures during the second winter. Our distribution model predicted an increased probability of Scaup use on larger ponds containing high densities of fish, while diet analysis indicated increased fish consumption during colder winter periods. Our results can be used by farm managers to designate resources for bird harassment to particular locations and times of the winter when scaup are more likely to negatively impact the fish crop